Papers › FISHing in Uncertainty: Synthetic Contrastive Learning for Genetic Aberration Detection

FISHing in Uncertainty: Synthetic Contrastive Learning for Genetic Aberration Detection

1 Nov 2024arXiv:2411.01025archive 2025-07-28

Simon Gutwein, Martin Kampel, Sabine Taschner-Mandl, Roxane Licandro

Detecting genetic aberrations is crucial in cancer diagnosis, typically through fluorescence in situ hybridization (FISH). However, existing FISH image classification methods face challenges due to signal variability, the need for costly manual annotations and fail to adequately address the intrinsic uncertainty. We introduce a novel approach that leverages synthetic images to eliminate the requirement for manual annotations and utilizes a joint contrastive and classification objective for training to account for inter-class variation effectively. We demonstrate the superior generalization capabilities and uncertainty calibration of our method, which is trained on synthetic data, by testing it on a manually annotated dataset of real-world FISH images. Our model offers superior calibration in terms of classification accuracy and uncertainty quantification with a classification accuracy of 96.7% among the 50% most certain cases. The presented end-to-end method reduces the demands on personnel and time and improves the diagnostic workflow due to its accuracy and adaptability. All code and data is publicly accessible at: https://github.com/SimonBon/FISHing

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ClassificationContrastive LearningDiagnosticImage ClassificationUncertainty Quantificationimage-classification

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