Papers › Using Deep Learning for Segmentation and Counting within Microscopy Data

Using Deep Learning for Segmentation and Counting within Microscopy Data

28 Feb 2018arXiv:1802.10548archive 2025-07-28

Carlos X. Hernández, Mohammad M. Sultan, Vijay S. Pande

Cell counting is a ubiquitous, yet tedious task that would greatly benefit from automation. From basic biological questions to clinical trials, cell counts provide key quantitative feedback that drive research. Unfortunately, cell counting is most commonly a manual task and can be time-intensive. The task is made even more difficult due to overlapping cells, existence of multiple focal planes, and poor imaging quality, among other factors. Here, we describe a convolutional neural network approach, using a recently described feature pyramid network combined with a VGG-style neural network, for segmenting and subsequent counting of cells in a given microscopy image.

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