Papers › Seeds Cleansing CNMF for Spatiotemporal Neural Signals Extraction of Miniscope Imaging Data

Seeds Cleansing CNMF for Spatiotemporal Neural Signals Extraction of Miniscope Imaging Data

3 Apr 2017arXiv:1704.00793links table onlyarchive 2025-07-28

Jinghao Lu, Chunyuan Li, Fan Wang

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Miniscope calcium imaging is increasingly being used to monitor large populations of neuronal activities in freely behaving animals. However, due to the high background and low signal-to-noise ratio of the single-photon based imaging used in this technique, extraction of neural signals from the large numbers of imaged cells automatically has remained challenging. Here we describe a highly accurate framework for automatically identifying activated neurons and extracting calcium signals from the miniscope imaging data, seeds cleansing Constrained Nonnegative Matrix Factorization (sc-CNMF). This sc-CNMF extends the conventional CNMF with two new modules: i) a neural enhancing module to overcome miniscope-specific limitations, and ii) a seeds cleansing module combining LSTM to rigorously select and cleanse the set of seeds for detecting regions-of-interest. Our sc-CNMF yields highly stable and superior performance in analyzing miniscope calcium imaging data compared to existing methods.

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