Papers › pgMAP: a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens
pgMAP: a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens
Phoebe C. R. Parrish, Daniel J. Groso, James D. Thomas, Robert K. Bradley, Alice H. Berger
We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts table and quality control metrics including the proportion of correctly-paired reads and CRISPR library sequencing coverage across all time points and samples. pgMAP is implemented using Snakemake and is available open-source under the MIT license at https://github.com/fredhutch/pgmap_pipeline.
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