Papers › On electron imaging of small molecules, Part II: tomographic reconstruction from defocus series
On electron imaging of small molecules, Part II: tomographic reconstruction from defocus series
A practical method utilising three-dimensional image pattern matching is proposed which, in principle, is capable of unambiguous determination of the types and positions of atoms in small molecules from defocus series collected at only a few different angular orientations of the molecule. Numerical tests of the method are presented using multislice calculations of defocus series of small biological molecules. The proposed technique can in future gain from Bayesian or machine-learning approaches and is likely to be useful in cryogenic electron microscopy.
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