Papers › Model-based aberration corrected microscopy inside a glass tube
Model-based aberration corrected microscopy inside a glass tube
D. W. S. Cox, T. Knop, I. M. Vellekoop
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Microscope objectives achieve near diffraction-limited performance only when used under the conditions they are designed for. In non-standard geometries, such as thick cover slips or curved surfaces, severe aberrations arise, inevitably impairing high-resolution imaging. Correcting such large aberrations using standard adaptive optics can be challenging: existing solutions are either not suited for strong aberrations, or require extensive feedback measurements, consequently taking a significant portion of the photon budget. We demonstrate that it is possible to pre-compute the corrections needed for high-resolution imaging inside a glass tube based on a priori information only. Our ray-tracing based method achieved over an order of magnitude increase in image contrast without the need for a feedback signal.
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