Papers › Microscopy Cell Segmentation via Convolutional LSTM Networks

Microscopy Cell Segmentation via Convolutional LSTM Networks

29 May 2018arXiv:1805.11247archive 2025-07-28

Assaf Arbelle, Tammy Riklin Raviv

Live cell microscopy sequences exhibit complex spatial structures and complicated temporal behaviour, making their analysis a challenging task. Considering cell segmentation problem, which plays a significant role in the analysis, the spatial properties of the data can be captured using Convolutional Neural Networks (CNNs). Recent approaches show promising segmentation results using convolutional encoder-decoders such as the U-Net. Nevertheless, these methods are limited by their inability to incorporate temporal information, that can facilitate segmentation of individual touching cells or of cells that are partially visible. In order to exploit cell dynamics we propose a novel segmentation architecture which integrates Convolutional Long Short Term Memory (C-LSTM) with the U-Net. The network's unique architecture allows it to capture multi-scale, compact, spatio-temporal encoding in the C-LSTMs memory units. The method was evaluated on the Cell Tracking Challenge and achieved state-of-the-art results (1st on Fluo-N2DH-SIM+ and 2nd on DIC-C2DL-HeLa datasets) The code is freely available at: https://github.com/arbellea/LSTM-UNet.git

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Code

arbellea/LSTM-UNet officialmentioned in papertf report
alvchn/fcn mentioned on GitHubtf report
ruveydayilmaz0/bvdm mentioned on GitHubpytorch report

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Tasks

Cell SegmentationCell TrackingSegmentation

Results from the paper archive 2025-07-28

TaskDatasetModelMetricValueRank at snapshotLeaderboardReport
Cell Segmentation DIC-C2DH-HeLa EncLSTM SEG (~Mean IoU) 0.793 #1 of 2 Archive leaderboard report
Cell Segmentation DIC-C2DH-HeLa DecLSTM SEG (~Mean IoU) 0.511 #2 of 2 Archive leaderboard report
Cell Segmentation Fluo-N2DH-GOWT1 DecLSTM SEG (~Mean IoU) 0.854 #1 of 2 Archive leaderboard report
Cell Segmentation Fluo-N2DH-GOWT1 EncLSTM SEG (~Mean IoU) 0.85 #2 of 2 Archive leaderboard report
Cell Segmentation Fluo-N2DH-SIM+ EncLSTM SEG (~Mean IoU) 0.811 #1 of 2 Archive leaderboard report
Cell Segmentation Fluo-N2DH-SIM+ DecLSTM SEG (~Mean IoU) 0.802 #2 of 2 Archive leaderboard report
Cell Segmentation Fluo-N2DL-HeLa DecLSTM SEG (~Mean IoU) 0.839 #2 of 3 Archive leaderboard report
Cell Segmentation Fluo-N2DL-HeLa EncLSTM SEG (~Mean IoU) 0.811 #3 of 3 Archive leaderboard report
Cell Segmentation PhC-C2DH-U373 EncLSTM SEG (~Mean IoU) 0.842 #1 of 1 Archive leaderboard report

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Methods

Concatenated Skip ConnectionConvolutionMax PoolingReLUU-Net

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