Papers › Cell identification in whole-brain multiview images of neural activation

Cell identification in whole-brain multiview images of neural activation

4 Nov 2015arXiv:1511.01168archive 2025-07-28

Marco Paciscopi, Ludovico Silvestri, Francesco Saverio Pavone, Paolo Frasconi

We present a scalable method for brain cell identification in multiview confocal light sheet microscopy images. Our algorithmic pipeline includes a hierarchical registration approach and a novel multiview version of semantic deconvolution that simultaneously enhance visibility of fluorescent cell bodies, equalize their contrast, and fuses adjacent views into a single 3D images on which cell identification is performed with mean shift. We present empirical results on a whole-brain image of an adult Arc-dVenus mouse acquired at 4micron resolution. Based on an annotated test volume containing 3278 cells, our algorithm achieves an F₁ measure of 0.89.

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