Papers › Application of signal separation to diffraction image compression and serial crystallography
Application of signal separation to diffraction image compression and serial crystallography
Jérôme Kieffer, Julien Orlans, Nicolas Coquelle, Samuel Debionne, Shibom Basu, Alejandro Homs, Gianluca Santonia, Daniele De Sanctis
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We present here a real-time analysis of diffraction images acquired at high frame-rate (925 Hz) and its application to macromolecular serial crystallography. The software uses a new signal separation algorithm, able to distinguish the amorphous (or powder diffraction) component from the diffraction signal originating from single crystals. It relies on the ability to work efficiently in azimuthal space and derives from the work performed on pyFAI, the fast azimuthal integration library. Two applications are built upon this separation algorithm: a lossy compression algorithm and a peak-picking algorithm; the performances of both is assessed by comparing data quality after reduction with XDS and CrystFEL.
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