{"about":{"site":"https://codewithpapers.app","non_affiliation":"Code with Papers and Syntology are not affiliated with, endorsed by, or sponsored by Papers with Code, Meta, or the pwc-archive mirror.","licence":"CC BY-SA 4.0","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","attribution":"https://codewithpapers.app/attribution","modified":"archive material modified by Syntology; see the attribution page"},"url":"/paper/zero-shot-denoising-for-fluorescence-lifetime","title":"Zero-Shot Denoising for Fluorescence Lifetime Imaging Microscopy with Intensity-Guided Learning","arxiv_id":"2503.13779","date":"2025-03-17","proceeding":null,"authors":["Hao Chen","Julian Najera","Dagmawit Geresu","Meenal Datta","Cody Smith","Scott Howard"],"abstract":"Multimodal and multi-information microscopy techniques such as Fluorescence Lifetime Imaging Microscopy (FLIM) extend the informational channels beyond intensity-based fluorescence microscopy but suffer from reduced image quality due to complex noise patterns. For FLIM, the intrinsic relationship between intensity and lifetime information means noise in each channel is a multivariate function across channels without necessarily sharing structural features. Based on this, we present a novel Zero-Shot Denoising Framework with an Intensity-Guided Learning approach. Our correlation-preserving strategy maintains important biological information that might be lost when channels are processed independently. Our framework implements separate processing paths for each channel and utilizes a pre-trained intensity denoising prior to guide the refinement of lifetime components across multiple channels. Through experiments on real-world FLIM-acquired biological samples, we show that our approach outperforms existing methods in both noise reduction and lifetime preservation, thereby enabling more reliable extraction of physiological and molecular information.","url_abs":"https://arxiv.org/abs/2503.13779v2","url_pdf":"https://arxiv.org/pdf/2503.13779v2.pdf","source":{"archive":"pwc-archive (Hugging Face), CC BY-SA 4.0","snapshot":"2025-07-28","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","row_kind":"abstracts"},"code_links":[{"paper_slug":"zero-shot-denoising-for-fluorescence-lifetime","repo_url":"https://github.com/haochen27/ZS-FLIM-Denoising","is_official":1,"mentioned_in_paper":0,"mentioned_in_github":0,"framework":"pytorch","reach":null}],"tasks":[{"task_slug":"denoising","task_name":"Denoising"}],"methods":[],"datasets_introduced":[],"methods_introduced":[],"results":[],"syntology":{"atlas_url":null,"mcp":null,"developers":"https://syntology.ai/developers"},"arxiv_metadata":null,"syntology_extracted_results":null}