{"about":{"site":"https://codewithpapers.app","non_affiliation":"Code with Papers and Syntology are not affiliated with, endorsed by, or sponsored by Papers with Code, Meta, or the pwc-archive mirror.","licence":"CC BY-SA 4.0","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","attribution":"https://codewithpapers.app/attribution","modified":"archive material modified by Syntology; see the attribution page"},"url":"/paper/label-free-prediction-of-three-dimensional","title":"Label-free prediction of three-dimensional fluorescence images from transmitted-light microscopy","arxiv_id":null,"date":"2018-09-17","proceeding":"Nature Methods 2018 9","authors":["Chawin Ounkomol","Sharmishtaa Seshamani","Mary M. Maleckar","Forrest Collman","Gregory R. Johnson"],"abstract":"Understanding cells as integrated systems is central to modern biology. Although fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, is slow, and can damage cells. We present a label-free method for predicting three-dimensional fluorescence directly from transmitted-light images and demonstrate that it can be used to generate multi-structure, integrated images. The method can also predict immunofluorescence (IF) from electron micrograph (EM) inputs, extending the potential applications.","url_abs":"https://pubmed.ncbi.nlm.nih.gov/30224672/","url_pdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212323/pdf/nihms-1501928.pdf","source":{"archive":"pwc-archive (Hugging Face), CC BY-SA 4.0","snapshot":"2025-07-28","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","row_kind":"abstracts"},"code_links":[{"paper_slug":"label-free-prediction-of-three-dimensional","repo_url":"https://github.com/allencellmodeling/pytorch_fnet","is_official":0,"mentioned_in_paper":1,"mentioned_in_github":0,"framework":"pytorch","reach":null}],"tasks":[{"task_slug":"image-to-image-translation","task_name":"Image-to-Image Translation"}],"methods":[],"datasets_introduced":[],"methods_introduced":[],"results":[],"syntology":{"syntology_url":null,"atlas_url":null,"mcp":null,"developers":"https://syntology.ai/developers"},"arxiv_metadata":null,"syntology_extracted_results":null}