{"about":{"site":"https://codewithpapers.app","non_affiliation":"Code with Papers and Syntology are not affiliated with, endorsed by, or sponsored by Papers with Code, Meta, or the pwc-archive mirror.","licence":"CC BY-SA 4.0","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","attribution":"https://codewithpapers.app/attribution","modified":"archive material modified by Syntology; see the attribution page"},"url":"/paper/fm2s-self-supervised-fluorescence-microscopy","title":"FM2S: Towards Spatially-Correlated Noise Modeling in Zero-Shot Fluorescence Microscopy Image Denoising","arxiv_id":"2412.10031","date":"2024-12-13","proceeding":null,"authors":["Jizhihui Liu","Qixun Teng","Qing Ma","Junjun Jiang"],"abstract":"Fluorescence microscopy image (FMI) denoising faces critical challenges due to the compound mixed Poisson-Gaussian noise with strong spatial correlation and the impracticality of acquiring paired noisy/clean data in dynamic biomedical scenarios. While supervised methods trained on synthetic noise (e.g., Gaussian/Poisson) suffer from out-of-distribution generalization issues, existing self-supervised approaches degrade under real FMI noise due to oversimplified noise assumptions and computationally intensive deep architectures. In this paper, we propose Fluorescence Micrograph to Self (FM2S), a zero-shot denoiser that achieves efficient FMI denoising through three key innovations: 1) A noise injection module that ensures training data sufficiency through adaptive Poisson-Gaussian synthesis while preserving spatial correlation and global statistics of FMI noise for robust model generalization; 2) A two-stage progressive learning strategy that first recovers structural priors via pre-denoised targets then refines high-frequency details through noise distribution alignment; 3) An ultra-lightweight network (3.5k parameters) enabling rapid convergence with 270$\\times$ faster training and inference than SOTAs. Extensive experiments across FMI datasets demonstrate FM2S's superiority: It outperforms CVF-SID by 1.4dB PSNR on average while requiring 0.1% parameters of AP-BSN. Notably, FM2S maintains stable performance across varying noise levels, proving its practicality for microscopy platforms with diverse sensor characteristics. Code and datasets will be released.","url_abs":"https://arxiv.org/abs/2412.10031v2","url_pdf":"https://arxiv.org/pdf/2412.10031v2.pdf","source":{"archive":"pwc-archive (Hugging Face), CC BY-SA 4.0","snapshot":"2025-07-28","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","row_kind":"abstracts"},"code_links":[{"paper_slug":"fm2s-self-supervised-fluorescence-microscopy","repo_url":"https://github.com/danielement321/fm2s","is_official":1,"mentioned_in_paper":1,"mentioned_in_github":1,"framework":"pytorch","reach":null}],"tasks":[{"task_slug":"computational-efficiency","task_name":"Computational Efficiency"},{"task_slug":"data-augmentation","task_name":"Data Augmentation"},{"task_slug":"denoising","task_name":"Denoising"},{"task_slug":"image-denoising","task_name":"Image Denoising"},{"task_slug":"out-of-distribution-generalization","task_name":"Out-of-Distribution Generalization"}],"methods":[],"datasets_introduced":[],"methods_introduced":[],"results":[],"syntology":{"atlas_url":"https://app.syntology.ai/?focus=2412.10031","mcp":null,"developers":"https://syntology.ai/developers"},"arxiv_metadata":null,"syntology_extracted_results":null}