{"about":{"site":"https://codewithpapers.app","non_affiliation":"Code with Papers and Syntology are not affiliated with, endorsed by, or sponsored by Papers with Code, Meta, or the pwc-archive mirror.","licence":"CC BY-SA 4.0","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","attribution":"https://codewithpapers.app/attribution","modified":"archive material modified by Syntology; see the attribution page"},"url":"/paper/d-sarcnet-a-dual-stream-deep-learning","title":"D-SarcNet: A Dual-stream Deep Learning Framework for Automatic Analysis of Sarcomere Structures in Fluorescently Labeled hiPSC-CMs","arxiv_id":"2410.14983","date":"2024-10-19","proceeding":null,"authors":["Huyen Le","Khiet Dang","Nhung Nguyen","Mai Tran","Hieu Pham"],"abstract":"Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are a powerful tool in advancing cardiovascular research and clinical applications. The maturation of sarcomere organization in hiPSC-CMs is crucial, as it supports the contractile function and structural integrity of these cells. Traditional methods for assessing this maturation like manual annotation and feature extraction are labor-intensive, time-consuming, and unsuitable for high-throughput analysis. To address this, we propose D-SarcNet, a dual-stream deep learning framework that takes fluorescent hiPSC-CM single-cell images as input and outputs the stage of the sarcomere structural organization on a scale from 1.0 to 5.0. The framework also integrates Fast Fourier Transform (FFT), deep learning-generated local patterns, and gradient magnitude to capture detailed structural information at both global and local levels. Experiments on a publicly available dataset from the Allen Institute for Cell Science show that the proposed approach not only achieves a Spearman correlation of 0.868 marking a 3.7% improvement over the previous state-of-the-art but also significantly enhances other key performance metrics, including MSE, MAE, and R2 score. Beyond establishing a new state-of-the-art in sarcomere structure assessment from hiPSC-CM images, our ablation studies highlight the significance of integrating global and local information to enhance deep learning networks ability to discern and learn vital visual features of sarcomere structure.","url_abs":"https://arxiv.org/abs/2410.14983v1","url_pdf":"https://arxiv.org/pdf/2410.14983v1.pdf","source":{"archive":"pwc-archive (Hugging Face), CC BY-SA 4.0","snapshot":"2025-07-28","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","row_kind":"abstracts"},"code_links":[{"paper_slug":"d-sarcnet-a-dual-stream-deep-learning","repo_url":"https://github.com/vinuni-vishc/d-sarcnet","is_official":1,"mentioned_in_paper":0,"mentioned_in_github":0,"framework":"pytorch","reach":null}],"tasks":[{"task_slug":"deep-learning","task_name":"Deep Learning"}],"methods":[{"method_slug":"mae","method_name":"MAE"}],"datasets_introduced":[],"methods_introduced":[],"results":[],"syntology":{"syntology_url":null,"atlas_url":null,"mcp":null,"developers":"https://syntology.ai/developers"},"arxiv_metadata":null,"syntology_extracted_results":null}