{"about":{"site":"https://codewithpapers.app","non_affiliation":"Code with Papers and Syntology are not affiliated with, endorsed by, or sponsored by Papers with Code, Meta, or the pwc-archive mirror.","licence":"CC BY-SA 4.0","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","attribution":"https://codewithpapers.app/attribution","modified":"archive material modified by Syntology; see the attribution page"},"url":"/paper/calculating-point-spread-functions-methods","title":"Calculating Point Spread Functions: Methods, Pitfalls and Solutions","arxiv_id":"2301.13515","date":"2023-01-31","proceeding":null,"authors":["Ratsimandresy Holinirina Dina Miora","Erich Rohwer","Martin Kielhorn","Colin J. R. Sheppard","Gurthwin Bosman","R. Heintzmann"],"abstract":"The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the centre position, yielding to the violation of the energy conservation. In this work, we present novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, and easy to implement and easy to modify to represent various imaging modalities.","url_abs":"https://arxiv.org/abs/2301.13515v2","url_pdf":"https://arxiv.org/pdf/2301.13515v2.pdf","source":{"archive":"pwc-archive (Hugging Face), CC BY-SA 4.0","snapshot":"2025-07-28","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","row_kind":"links_only","authors_date_abstract":"arXiv metadata, CC0 1.0 (https://info.arxiv.org/help/license), from the Kaggle arXiv metadata snapshot of 2026-09-12"},"code_links":[{"paper_slug":"calculating-point-spread-functions-methods","repo_url":"https://github.com/bionanoimaging/psftoolbox-matlab","is_official":1,"mentioned_in_paper":1,"mentioned_in_github":0,"framework":"none","reach":null}],"tasks":[],"methods":[],"datasets_introduced":[],"methods_introduced":[],"results":[],"syntology":{"atlas_url":null,"mcp":null,"developers":"https://syntology.ai/developers"},"arxiv_metadata":null,"syntology_extracted_results":null}