{"about":{"site":"https://codewithpapers.app","non_affiliation":"Code with Papers and Syntology are not affiliated with, endorsed by, or sponsored by Papers with Code, Meta, or the pwc-archive mirror.","licence":"CC BY-SA 4.0","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","attribution":"https://codewithpapers.app/attribution","modified":"archive material modified by Syntology; see the attribution page"},"url":"/paper/apple-picker-automatic-particle-picking-a-low","title":"APPLE Picker: Automatic Particle Picking, a Low-Effort Cryo-EM Framework","arxiv_id":"1802.00469","date":"2018-02-01","proceeding":null,"authors":["Ayelet Heimowitz","Joakim andén","Amit Singer"],"abstract":"Particle picking is a crucial first step in the computational pipeline of\nsingle-particle cryo-electron microscopy (cryo-EM). Selecting particles from\nthe micrographs is difficult especially for small particles with low contrast.\nAs high-resolution reconstruction typically requires hundreds of thousands of\nparticles, manually picking that many particles is often too time-consuming.\nWhile semi-automated particle picking is currently a popular approach, it may\nsuffer from introducing manual bias into the selection process. In addition,\nsemi-automated particle picking is still somewhat time-consuming. This paper\npresents the APPLE (Automatic Particle Picking with Low user Effort) picker, a\nsimple and novel approach for fast, accurate, and fully automatic particle\npicking. While our approach was inspired by template matching, it is completely\ntemplate-free. This approach is evaluated on publicly available datasets\ncontaining micrographs of $\\beta$-galactosidase and keyhole limpet hemocyanin\nprojections.","url_abs":"http://arxiv.org/abs/1802.00469v2","url_pdf":"http://arxiv.org/pdf/1802.00469v2.pdf","source":{"archive":"pwc-archive (Hugging Face), CC BY-SA 4.0","snapshot":"2025-07-28","licence_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","row_kind":"abstracts"},"code_links":[{"paper_slug":"apple-picker-automatic-particle-picking-a-low","repo_url":"https://github.com/PrincetonUniversity/APPLEpicker","is_official":1,"mentioned_in_paper":1,"mentioned_in_github":1,"framework":"none","reach":{"status":"unanswered"}}],"tasks":[{"task_slug":"template-matching","task_name":"Template Matching"}],"methods":[],"datasets_introduced":[],"methods_introduced":[],"results":[],"syntology":{"atlas_url":"https://app.syntology.ai/?focus=1802.00469","mcp":null,"developers":"https://syntology.ai/developers"},"arxiv_metadata":null,"syntology_extracted_results":null}