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Subsequently, AO is then tested on the classical IEEE CEC 2014, and the latest IEEE CEC 2022 benchmark function sets to assess its adaptability. Comparative analyses are conducted against eight well-established algorithms and eight high-performance improved algorithms. Statistical analyses of convergence curves and qualitative metrics revealed AO's robust competitiveness. Lastly, the AO is incorporated into breast cancer pathology image segmentation applications. Using 15 authentic medical images at six threshold levels, AO's segmentation performance is compared against eight distinguished algorithms. Experimental results demonstrated AO's superiority in terms of image segmentation accuracy, Feature Similarity Index (FSIM), Peak Signal-to-Noise Ratio (PSNR), and Structural Similarity Index (SSIM) over the contrast algorithms. These results emphasize AO's efficiency and its potential in real-world optimization applications. 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