{"url":"/dataset/paviaatn","name":"PaviaATN","full_name":null,"description_markdown":"The PaviaATN data consists of 62 4-channel fluorescence microscopy images of size 2720 × 2720. The four channels, in order, label Nuclei (first two), Actin and Tubulin. It was imaged in the Synthetic Physiology Laboratory(https://www.syntheticphysiologylab.com/) of the University of Pavia, and introduced in μSplit: image decomposition for fluorescence microscopy(https://arxiv.org/abs/2211.12872), published at ICCV 2023.\r\n\r\nDetailed Description\r\nThe PaviaATN dataset comprises static lambda-stacks from a human keratinocytes cell line (HaCaT) expressing GFP-tubulin, RFP-LifeAct, and a customized version of the cell cycle indicator FastFUCCI that uses various combinations of a yellow fluorescent protein (YPF, mTurquoise2) and a far-red fluorescent protein (iRFP, miRFP670) to indicate multiple phases of the cell cycle. \r\n\r\nWhen a cell is in the G1 phase, increasing intensities of YFP fluorescence are detected in the nucleus. As a cell moves from G1 to S phase (G1/S), both YFP and iRFP fluorescence are detected in the nucleus of the cell. \r\nFinally, the sole iRFP fluorescence is detected in the nucleus during the S-G2-M phase. At the onset of the G1 phase, the nucleus shows no visible fluorescence intensity. In [μSplit: image decomposition for fluorescence microscopy](https://arxiv.org/abs/2211.12872), this dataset was used to resolve overlapping structures.\r\n\r\nAll Images were acquired in a Nikon Ti2 microscope (100x silicon oil objective) equipped with an Okolab environmental control chamber and a Crest V3 spinning disk confocal in widefield mode.","description_withheld":null,"homepage":"https://zenodo.org/records/8235843","introduced_date":"2022-11-23","introduced_date_note":null,"introduced_by":{"paper":"/paper/msplit-efficient-image-decomposition-for","title":"μSplit: efficient image decomposition for microscopy data","first_author":"Ashesh","url":null},"license":null,"modalities":[],"tasks":[],"languages":[],"variants":["PaviaATN"],"data_loaders":[],"num_papers_in_archive":1,"source":{"archive":"pwc-archive (Hugging Face), CC BY-SA 4.0","snapshot":"2025-07-28"},"benchmarks":[],"papers_with_a_benchmark_row":[],"syntology_totals":{"read_at":"2026-09-24T18:15:14+00:00","papers_with_samples":0,"samples_harvested":0,"samples_ran":0,"samples_unverified":0,"pointer_only_for_licence":0,"papers_with_no_sample_that_ran":0,"note":"the per-paper counts above, summed; not a rate"},"papers_note":"The archive never published its papers-using-dataset list; these are papers with a leaderboard row on this dataset's benchmarks."}